Biotechnology and bioengineering · 2021
Rapid and cost-effective process based on insect larvae for scale-up production of SARS-COV-2 spike protein for serological COVID-19 testing.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Smith Ignacio, Mc Callum Gregorio Juan, Sabljic Adriana Victoria, Marfia Juan Ignacio, Bombicino Silvina Sonia, Trabucchi Aldana, Iacono Ruben Francisco, Birenbaum Joaquín Manuel, Vazquez Susana Claudia, Minoia Juan Mauricio, Cascone Osvaldo, López María Gabriela, Taboga Oscar, Targovnik Alexandra Marisa, Wolman Federico Javier, Fingermann Matías, Alonso Leonardo Gabriel, Valdez Silvina Noemí, Miranda María Victoria
Аннотация
Serology testing for COVID-19 is important in evaluating active immune response against SARS-CoV-2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus-exposed individuals and vaccine-mediated immunity. In this study, recombinant S protein of SARS-CoV-2 was expressed in Rachiplusia nu, an important agronomic plague. One gram of insect larvae produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS-CoV-2 S protein that showed immunoreactivity for anti-SARS-CoV-2 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost-effective platform for large-scale S protein production, and the scale-up is linear, thus avoiding the use of complex equipment like bioreactors.