Structural characteristics of the kidney in conditions of experimental hypothermia.

OBJECTIVE

Aim: To investigate histological, ultrastructural, and morphometric changes in the wall of arterial vessels, glomerular and peritubular capillaries, and convoluted tubules of the kidney under the influence of a cold factor in an experiment.

PATIENTS AND

METHODS

Materials and

Methods

The experiment was conducted on 80 mature white Wistar male rats, 10 of which served as controls. Animals were divided into 7 groups of 10 animals each according to experimental time points: at the peak of cooling and at 1, 3, 7, 14, 21, and 30 days of the recovery period. All procedures with animals were performed according to bioethical standards. Experimental-morphological analysis was conducted using histological, electron microscopic, and morphometric research methods.

RESULTS

Results

It was established that hypothermia causes vasoconstriction of the kidney's arterial vessels. From day 1 of the post-hypothermic period, edematous changes develop, which increase maximally by day 3 and persist through day 7 of the experiment. Starting from day 14, edematous changes decrease and structural organization of kidney parenchyma is restored. Reparative processes are completed by day 30.

CONCLUSION

Conclusion

Profound renal hypothermia causes a complex process: early vascular spasm leading to ischemia, subsequent inflammatory enhancement of damage with maximum effect at days 3-7, followed predominantly by reversible recovery with isolated foci of irreversible remodeling.