F&S science · 2026
Testicular sperm extraction prediction for patients with nonobstructive azoospermia by Raman spectroscopy.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Aleksandrov Artem, Rimskaya Elena, Gorevoy Alexey, Lobanova Nataliia, Borisova Nataliya, Makarova Natalia, Chernyshev Vasiliy, Gorin Dmitry, Sukhikh Gennady
Аннотация
OBJECTIVE: To address the need for a noninvasive test to predict testicular sperm extraction (TESE) success for patients with nonobstructive azoospermia (NOA).
DESIGN: Retrospective case-control study.
SUBJECTS: A total of 20 men aged 29-50 years with NOA who underwent microdissection TESE (microTESE) procedure were divided into positive [TESE(+), N = 10] and negative [TESE(-), N = 10] categories based on the success of sperm retrieval. Seminal plasma samples were obtained before the surgery and used in the study after the microTESE outcome became known.
EXPOSURE: Raman spectral features of seminal plasma samples in 2,800-3,050 cm-1 wavenumber range obtained with hollow-core microstructured optical fiber (HC-MOF); nuclear magnetic resonance spectral features; reactive oxygen species (ROS) level measured using enzyme-linked immunosorbent assay (ELISA).
MAIN OUTCOME MEASURES: Successive sperm extraction by the microTESE procedure.
RESULTS: Raman spectroscopy analysis enhanced by HC-MOF revealed characteristic spectral differences between TESE(+) and TESE(-) seminal plasma samples in 2,850, 2,875, and 2,920 cm-1 bands related to C-H stretching vibrations, which may be associated with an elevated contribution from lipids in TESE(+) samples. The diagnostic model based on principal component analysis provided 79%/68% accuracy, 0.863/0.810 receiver operating characteristic area under curve, and 77%/66% specificity with 80%/71% sensitivity (13-19/5-12 diagnostic odds ratio) on the training/test set using 5-fold 10-iteration cross-validation. The statistical significance of higher ROS levels in TESE(-) samples was not enough for robust prediction of successful TESE.
CONCLUSION: The HC-MOF-enhanced Raman spectroscopy is a rapid, noninvasive, cost-efficient, and easy-to-use method, which can be applied to predict TESE success in men with NOA, revealing differences in biochemical profiles of seminal plasma. Although validation on a bigger cohort is needed, this proof-of-concept study highlights perspectives of the technique for its widespread use in presurgical screening, preventing the risky invasive procedure in the case of low sperm extraction chance.
DESIGN: Retrospective case-control study.
SUBJECTS: A total of 20 men aged 29-50 years with NOA who underwent microdissection TESE (microTESE) procedure were divided into positive [TESE(+), N = 10] and negative [TESE(-), N = 10] categories based on the success of sperm retrieval. Seminal plasma samples were obtained before the surgery and used in the study after the microTESE outcome became known.
EXPOSURE: Raman spectral features of seminal plasma samples in 2,800-3,050 cm-1 wavenumber range obtained with hollow-core microstructured optical fiber (HC-MOF); nuclear magnetic resonance spectral features; reactive oxygen species (ROS) level measured using enzyme-linked immunosorbent assay (ELISA).
MAIN OUTCOME MEASURES: Successive sperm extraction by the microTESE procedure.
RESULTS: Raman spectroscopy analysis enhanced by HC-MOF revealed characteristic spectral differences between TESE(+) and TESE(-) seminal plasma samples in 2,850, 2,875, and 2,920 cm-1 bands related to C-H stretching vibrations, which may be associated with an elevated contribution from lipids in TESE(+) samples. The diagnostic model based on principal component analysis provided 79%/68% accuracy, 0.863/0.810 receiver operating characteristic area under curve, and 77%/66% specificity with 80%/71% sensitivity (13-19/5-12 diagnostic odds ratio) on the training/test set using 5-fold 10-iteration cross-validation. The statistical significance of higher ROS levels in TESE(-) samples was not enough for robust prediction of successful TESE.
CONCLUSION: The HC-MOF-enhanced Raman spectroscopy is a rapid, noninvasive, cost-efficient, and easy-to-use method, which can be applied to predict TESE success in men with NOA, revealing differences in biochemical profiles of seminal plasma. Although validation on a bigger cohort is needed, this proof-of-concept study highlights perspectives of the technique for its widespread use in presurgical screening, preventing the risky invasive procedure in the case of low sperm extraction chance.