Neuroscience bulletin · 2025
Multifaceted Role of RIMBP2 in Promoting Hearing in Murine Cochlear Hair Cells.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Liao Menghui, Chen Xin, Lu Ling, Liu Qing, Guo Rongrong, Qiu Yuyang, Hu Yangnan, Zhang Yuhua, Fang Qiaojun, Zhang Panpan, Li Yige, He Shuijin, Tang Mingliang, Li Huawei, Li Geng-Lin, Chai Renjie
Аннотация
The mammalian cochlea relies on outer and inner hair cells (OHCs/IHCs) for sound amplification and signal transmission. Rab3-interacting molecular binding protein 2 (RIMBP2), expressed in receptor cells and neurons at synaptic active zones, remains poorly characterized in hearing. We therefore generated a Rimbp2 knockout (KO) mouse model (Rimbp2-/-), which exhibited severe hearing loss with elevated thresholds, prolonged latencies, and reduced amplitudes in auditory brainstem response Wave I. OHC loss via apoptosis was correlated with threshold elevation. In IHCs, patch-clamp recordings revealed reduced exocytosis, including a diminished readily-releasable pool, impaired sustained release, and blocked fast endocytosis. Immunostaining showed unchanged ribbon synapse numbers but positional shifts in the basal pole of KO IHCs. These findings demonstrated RIMBP2's essential role in OHC survival and its broader regulatory functions in IHC synaptic transmission than previously recognized.