The Anti-atopic Perilla Seed Oil and Ethanol Extract on TNF-α/IFN-γ-Stimulated inflammatory Responses in Human HaCaT Keratinocytes.

Chronic inflammatory atopic dermatitis (AD) is a skin disorder characterized by cutaneous inflammation and a dysfunctional skin barrier. Although perilla seed oil (PO) is known to exhibit various biological activities, including anti-cancer, anti-inflammatory, and anti-oxidant effects, its inhibitory effects together with the ethanol extract of PO (PE) on atopic and inflammatory responses in the dermal skin has not yet been investigated. In the present study, PE significantly reduced mRNA expressions of inflammation-associated cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β, IL-6, IL-8, and IL-33. Furthermore, PE decreased the levels of the chemokine, thymus- and activation-regulated chemokine (TARC/CCL17) in TNF-α and interferon (IFN)-γ-stimulated human HaCaT keratinocytes. In addition, PE effectively suppressed activation of MAPK/NF-κB and JAK/STAT signaling pathways in HaCaT keratinocytes co-stimulated with TNF-α and IFN-γ. These findings indicate that PE possesses anti-inflammatory and anti-atopic properties, suggesting its potential benefits in treating inflammatory skin diseases including AD.