Molecular biology reports · 2026
Antioxidant enhancement and myopia progression delay by Rho-kinase inhibition in guinea pig retina.
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Liu Yijie, Hao Qi, Lu Xiuzhen, Wang Pubo, Guo Dadong, Pan Xuemei, Wu Qiuxin, Bi Hongsheng
Аннотация
BACKGROUND: Myopia is a common ocular condition that threatens the vision of children and adolescents. This study aimed to investigate the effect of the Rho-kinase inhibitor Y27632 on retinal oxidative stress, tissue structure, and myopia progression in guinea pigs with lens-induced myopia.
METHODS AND RESULTS: The animals were randomly assigned to five groups: normal control, lens-induced myopia (LIM), Y27632 low-dose (LD), Y27632 medium-dose (MD), and Y27632 high-dose (HD). Refraction was measured by retinoscopy, axial length (AL) was determined using A-scan ultrasonography, and retinal tissue morphology was examined by hematoxylin and eosin staining. The mRNA and protein expression levels of related factors were analyzed using quantitative polymerase chain reaction and Western blotting. Retinal levels of catalase, glutathione, superoxide dismutase, and malondialdehyde (MDA) were measured using an enzyme-linked assay. The results showed that, compared with the LIM group, guinea pigs in the LD, MD, and HD groups exhibited reduced myopic diopters, slower AL elongation, significantly increased retinal cell counts, decreased expression of Rho/ROCK pathway-related factors, a lower matrix metalloproteinase/tissue inhibitor of metalloproteinase ratio, and enhanced antioxidant activity.
CONCLUSIONS: These findings suggest that the Rho-kinase inhibitor Y27632 may regulate Rho/ROCK signaling in the retina of guinea pigs with lens-induced myopia, improve the retinal microenvironment, and delay the progression of myopia.
METHODS AND RESULTS: The animals were randomly assigned to five groups: normal control, lens-induced myopia (LIM), Y27632 low-dose (LD), Y27632 medium-dose (MD), and Y27632 high-dose (HD). Refraction was measured by retinoscopy, axial length (AL) was determined using A-scan ultrasonography, and retinal tissue morphology was examined by hematoxylin and eosin staining. The mRNA and protein expression levels of related factors were analyzed using quantitative polymerase chain reaction and Western blotting. Retinal levels of catalase, glutathione, superoxide dismutase, and malondialdehyde (MDA) were measured using an enzyme-linked assay. The results showed that, compared with the LIM group, guinea pigs in the LD, MD, and HD groups exhibited reduced myopic diopters, slower AL elongation, significantly increased retinal cell counts, decreased expression of Rho/ROCK pathway-related factors, a lower matrix metalloproteinase/tissue inhibitor of metalloproteinase ratio, and enhanced antioxidant activity.
CONCLUSIONS: These findings suggest that the Rho-kinase inhibitor Y27632 may regulate Rho/ROCK signaling in the retina of guinea pigs with lens-induced myopia, improve the retinal microenvironment, and delay the progression of myopia.