[Research on anti-4T1 mouse breast cancer efficacy of injectable liposome hydrogel co-loaded with gambogic acid and indocyanine green].

This study aimed to assess the antitumor efficacy of a liposome hydrogel(GA-ICG-Lip-gel) co-loaded with gambogic acid(GA) and indocyanine green(ICG) both in vitro and in vivo, in combination with near infrared(NIR). The biological safety of GA-ICG-Lip-gel was assessed through the hemolysis assay. The inhibitory effects of various formulations, alone and in combination with 808 nm(2.5 W·cm~(-2)) NIR irradiation, on tumor cells were evaluated using the CCK-8 assay. A BALB/c mouse model with 4T1 breast cancer was established. In vivo photothermal properties of the nanocomposite system were assessed via infrared thermal imaging. The anticancer efficacy of GA-ICG-Lip-gel was evaluated through pharmacodynamic testing, hematoxylin-eosin(HE) staining, TdT-mediated dUTP Nick-end labeling(TUNEL) of tumor tissue, and Ki67 immunohistochemistry for proliferation cell-associated antigen. Interleukin-6(IL-6) levels were measured using an ELISA assay, along with tumor necrosis factor-α(TNF-α) and interferon-γ(IFN-γ) levels.

RESULTS

: indicate that GA-ICG-Lip-gel exhibits excellent biological safety and photothermal stability, with a concentration-and time-dependent inhibitory effect on 4T1 cells. The cellular uptake of GA-ICG-Lip-gel by 4T1 cells is highly efficient, leading to a significant suppression of their migratory capacity. In vivo, GA-ICG-Lip-gel exhibits excellent photothermal conversion properties and effectively inhibits the growth of 4T1 breast cancer cells. ELISA assay results indicate that GA-ICG-Lip-gel + NIR notably increases the levels of IL-6, TNF-α, and IFN-γ in tumor-bearing mice. In summary, GA-ICG-Lip-gel serves as a promising nanodrug delivery platform that, when combined with NIR irradiation, displays potent antitumor effects both in vitro and in vivo.