Microbial pathogenesis · 2025
Detection of glycopeptide resistance and virulence genes in enterococci isolated from cheese and investigation of the clonal relationship of E. faecium species with rectal surveillance isolates.
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Pembe Büşra, Orak Filiz, Karabekmez Erdem Tuğba, Yalçınkaya Kezban Tülay, Doğaner Adem
Аннотация
OBJECTIVE: This study aimed to investigate the presence of glycopeptide resistance and virulence genes in Enterococcus spp. isolated from cheese and the clonal relationship of E. faecium species with rectal surveillance isolates.
MATERIALS AND METHOD: The study included 50 E. faecium species identified by the BD Phoenix 100 automatic identification system from surveillance cultures sent to the Kahramanmaraş Sütçü İmam University's microbiology laboratory and Enterococcus species from various packaged and unpackaged cheese samples. Multiplex polymerase chain reaction was used to investigate the presence of glycopeptide resistance and virulence genes in enterococci isolates. The clonal relationship between E. faecium isolates was evaluated using the Enterobacterial Repetitive Intergenic Consensus polymerase chain reaction method.
RESULTS: Bacterial growth was detected in 33 of the cheese samples. The study included E. faecium and E. faecalis species. Glycopeptide resistance genes were detected in 11 E. faecalis isolates, including vanD (61.1 %) and vanB (44.4 %). None of the glycopeptide resistance genes were detected in E. faecium isolates. Additionally, virulence genes were detected in 5 (31.25 %) E. faecium and 12 (66.6 %) E. faecalis isolates. Glycopeptide resistance genes were more common in isolates from packaged cheeses (p = 0.045).
CONCLUSION: Four E. faecium strains isolated from cheeses were closely related to the rectal surveillance isolates. This suggests that the health risks associated with enterococci in cheese must be carefully analyzed.
MATERIALS AND METHOD: The study included 50 E. faecium species identified by the BD Phoenix 100 automatic identification system from surveillance cultures sent to the Kahramanmaraş Sütçü İmam University's microbiology laboratory and Enterococcus species from various packaged and unpackaged cheese samples. Multiplex polymerase chain reaction was used to investigate the presence of glycopeptide resistance and virulence genes in enterococci isolates. The clonal relationship between E. faecium isolates was evaluated using the Enterobacterial Repetitive Intergenic Consensus polymerase chain reaction method.
RESULTS: Bacterial growth was detected in 33 of the cheese samples. The study included E. faecium and E. faecalis species. Glycopeptide resistance genes were detected in 11 E. faecalis isolates, including vanD (61.1 %) and vanB (44.4 %). None of the glycopeptide resistance genes were detected in E. faecium isolates. Additionally, virulence genes were detected in 5 (31.25 %) E. faecium and 12 (66.6 %) E. faecalis isolates. Glycopeptide resistance genes were more common in isolates from packaged cheeses (p = 0.045).
CONCLUSION: Four E. faecium strains isolated from cheeses were closely related to the rectal surveillance isolates. This suggests that the health risks associated with enterococci in cheese must be carefully analyzed.