Цитометрический анализ активации клеток мыши CD8+T.
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Nguyen Du Huy, Thiem Manja Wibke, Veltkamp Alexander Winston, Schädlich Ines Sophie, Jiang Jiani, Panda Mamta, Costanzo Enrico Maria, Magnus Tim, Rissiek Björn
Аннотация
Оригинал публикации
Flow Cytometric Analysis of Mouse Brain CD8+ T Cell Activation.
Cytotoxic CD8+ T cell lymphocytes are pivotal for fighting infections with intracellular pathogens, e.g., viruses. Moreover, recent studies have elucidated the role of CD8+ T cells in various neuroinflammatory diseases such as Alzheimer's disease (AD), Parkinson's disease (PD), multiple sclerosis (MS), and stroke. Therefore, the characterization of brain CD8+ T cells at the single-cell level is pivotal for understanding their state and function, e.g., in preclinical mouse models. Here, flow cytometry emerges as a versatile tool offering distinct techniques tailored to different facets of T cell activation. In this chapter, we provide protocols utilizing multicolor flow cytometry and cytokine secretion assays to analyze brain CD8+ T cell activation via the detection of surface lineage markers, extracellular and intracellular activation markers such as CD39, CD279, and granzyme B, or via measuring cytokine expression levels.