Injectable hydrogels loaded with EGCG /KGN for the treatment of osteoarthritis.

Osteoarthritis is confronted with a multifaceted pathological microenvironment, and the implementation of palliative pharmacological strategies facilitates the continued progression of osteoarthritis. In this study, a dynamic hydrogel composed of oxidized hyaluronic acid and gelatin was devised for encapsulating and dispensing EGCG to combat inflammation and oxidative stress in the initial phase, stimulating the differentiation of BMSCs to preserve the metabolic equilibrium of the extracellular matrix through the gradual release of KGN-loaded PLGA microspheres. Subsequently, accomplished adhesion and in-situ delivery were achieved through minimally invasive injection into the joint cavity. These hydrogels possess excellent shear-thinning properties and biocompatibility. The design of double-loaded hydrogel is capable of eradicating intracellular reactive oxygen species while fostering cartilage differentiation via controlled release of EGCG and KGN. A double-loaded injectable hydrogel may provide a new idea for early minimally invasive treatment of osteoarthritis.