[Chemical constituents from fruits of Morus nigra distributed in Xinjiang and their inhibitory effects on proliferation of synovial fibroblasts].

The chemical constituents from the fruits of Morus nigra distributed in Xinjiang were investigated by various chromatographic separation methods, including silica gel column chromatography, reversed-phase silica gel column chromatography, Sephadex LH-20 gel column chromatography, and preparative high-performance liquid chromatography. Based on the physicochemical properties and spectroscopic data of the isolated compounds, as well as literature data, 15 compounds were identified as morusnigrane A(1), 4-hydroxylonchocarpin(2), isobavachalcone(3), euchrenone a7(4), apigenin(5), medicarpin(6),(3R)-vestitol(7), pinnatifidanin CⅡ(8), pinnatifidanin CⅠ(9),(+)-lyoniresinol(10), artotonkin(11), resveratrol(12), 4-hydroxycinnamic acid(13), myrrhain A(14), and 2,5-di-tert-butylphenol(15). Compound 1 was a new 2-arylbenzofuran, and compounds 7-9, 14, and 15 were isolated from the genus Morus for the first time. 2-Arylbenzofurans 1 and 11, flavonoids 2-4, lignans 8 and 9, and phenolic acid 14 showed inhibitory effects on the proliferation of MH7A synovial fibroblasts with the IC_(50) values ranging from(5.17±0.05) to(152.97±0.20) μmol·L~(-1), displaying the potential of anti-rheumatoid arthritis.