Self-assembled near-infrared-photothermal antibacterial Hericium erinaceus β-glucan/tannic acid/Fe (III) hydrogel for accelerating infected wound healing.

Bacterial infection severely hinders skin wound healing, highlighting the critical application value of developing antibacterial and anti-inflammatory hydrogel dressings. In this work, we focused on β-glucan from Hericium erinaceus (HEBG) as the research object, and proposed a solvent-induced combined temperature manipulation technique to trigger multilevel self-assembly of β-glucan. Furthermore, we incorporated green synthesized near-infrared photosensitizer tannic acid (TA)/iron (III) complex into the system. A hydrogel with exceptional antibacterial properties, capable of responding to near-infrared photothermal stimuli while exhibiting remarkable stiffness and structural consistency, was successfully synthesized. Under near-infrared radiation, HEBG/TA/Fe hydrogels produced local hyperthermia and exhibited excellent antibacterial activity against bacteria-infected wounds. Moreover, the HEBG/TA/Fe hydrogel demonstrates its ability to regulate cytokines by effectively inhibiting the production of inflammatory mediators TNF-α and IL-6, while simultaneously enhancing the expression of cell proliferation factor KI-67 and markers associated with angiogenesis such as CD31 and α-SMA. Notably, the results of tissue staining revealed that the NIR + HEBG/TA/Fe5 hydrogel could effectively promoting granulation and vascularization, improving collagen deposition in infected wounds thereby accelerating the healing process. These findings indicate that mixed hydrogels exhibit potential as viable options for the treatment of bacterial infections.