EXPLORING THE MECHANISM OF ACTION OF HEMP SEEDS (CANNABIS SATIVA L.) IN TREATING OSTEOPOROSIS USING NETWORK PHARMACOLOGY.

This study's objective was to elucidate the molecular mechanism of hemp seeds against osteoporosis using a network pharmacology approach. The methodology entailed the retrieval of active components and targets of hemp seeds from the TCMSP database, as well as OP-related targets from GeneCards, TTD, Drugbank, and OMIM databases, followed by identifying their intersection. Network construction and enrichment analyses were performed using Cytoscape and Metascape. Finally, molecular docking was utilized to validate the binding capacity between the core active ingredients and the key targets. As a result, six active components and 96 potential targets of hemp seeds were identified, which intersected with 1,745 OP-related targets to yield 53 common targets. GO enrichment analysis revealed that these targets were primarily involved in biological processes such as inflammatory response, while KEGG analysis highlighted significant pathways including the AGE-RAGE and PI3K-Akt signaling pathways. Notably, a subsequent analysis of the protein-protein interaction (PPI) network topology identified AKT1, TNF, and IL6 as pivotal targets within the therapeutic mechanism. In summary, the present study initially revealed the potential mechanism of OP treatment with hemp seeds and provided a reference for conducting experimental studies.