Anti-resorptive dihydrophenanthrene coelonin alleviates arthritic bone destruction by inhibiting osteoclast formation and synovial inflammation through Src regulation.

BACKGROUND

The overactivation of osteoclasts (OCs) and fibroblast-like synoviocytes (FLS) is a key contributor to the bone destruction associated with rheumatoid arthritis (RA). Coelonin (COE) is known as an anti-inflammatory dihydrophenanthrene, but its anti-arthritic properties, direct targets, and potential mechanisms remain to be elucidated.

PURPOSE: This study aims to investigate the mitigating effect and molecular mechanisms of COE on RA-associated bone destruction.

METHODS

The effects of COE on OC formation and FLS activation were evaluated using the RANKL-induced OC differentiation model and IL-1β-stimulated SW982 cells, respectively. In vivo anti-arthritic effect of COE was evaluated in collagen-induced arthritis (CIA) mice. Network pharmacology was applied for the target identification of COE, followed by the multi-level target validation and elucidation of drug-target binding modes.

RESULTS

COE administration significantly inhibited RANKL-induced differentiation and maturation of OCs, and blocked IL-1β-induced inflammatory activation of FLS. Such dual regulatory activities of COE provided a significant contribution to the alleviation of arthritic progression and bone destruction in CIA mice. Using network pharmacology analysis and subsequent target validation, Src kinase was identified as the critical target of COE responsible for its anti-arthritic effect. Mechanistically, COE directly interacted with Src, and the hydrogen bonds established within the kinase domain were essential for the binding of COE to Src. COE inhibited the phosphorylation activation of Src and downstream MAPK and PI3K/AKT/GSK3β signaling pathways, ultimately leading to significant inhibition of arthritic progression and alleviation of bone destruction.

CONCLUSIONS

These findings revealed for the first time the anti-arthritic effects of COE, and suggested that COE could be a promising dual-function candidate for RA treatment by targeting Src kinase to inhibit synovial inflammation and bone erosion.