Молекулярное профилирование мозговой эндотелиальной клетки для астроцитной связи между мышью и человеком.
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Hill Steven A, Bravo-Ferrer Isabel, Čiulkinytė Austėja, Pérez Ramos Noelia, Rossetti Ilaria, Colvin Chiara, Beltran-Lobo Paula, Parra-Pérez Carlos, Emelianova Katie, Dando Owen, Geary Beth, Nirujogi Raja S, Alessi Dario R, Lee Do-Young, Lee Youn-Bok, Díaz Castro Blanca
Аннотация
Оригинал публикации
Molecular profiling of brain endothelial cell to astrocyte endfoot communication in mouse and human.
Our understanding of how the body communicates with the brain to coordinate their functions is remarkably limited. At the blood-brain barrier (BBB), brain endothelial cells (BECs) are ideally positioned to mediate signaling between blood and brain parenchyma via direct communication with astrocyte perivascular processes (endfeet). We develop a method to define the mouse in vivo astrocyte endfoot proteome, which in combination with BEC-specific RNA-seq, reveal BEC to astrocyte endfoot ligand-receptor pairs that are modulated when mice are exposed to a peripheral inflammatory insult with lipopolysaccharide. We show that over 80% of these mouse BEC-endfoot ligand-receptor pairs are also found in the human BBB, with a subset of them differentially expressed in human multiple sclerosis or Alzheimer's disease compared to healthy individuals. Our findings reveal dynamic BEC-endfoot communication pathways that are relevant to human physiology and provide methodology and datasets for the translational study of BEC-astrocyte crosstalk in health and disease.