Cell reports · 2017
Cell Type of Origin Dictates the Route to Pluripotency.
СтатьяНазвание и аннотация: Перевод в очереди
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Nefzger Christian M, Rossello Fernando J, Chen Joseph, Liu Xiaodong, Knaupp Anja S, Firas Jaber, Paynter Jacob M, Pflueger Jahnvi, Buckberry Sam, Lim Sue Mei, Williams Brenda, Alaei Sara, Faye-Chauhan Keshav, Petretto Enrico, Nilsson Susan K, Lister Ryan, Ramialison Mirana, Powell David R, Rackham Owen J L, Polo Jose M
Аннотация
Our current understanding of induced pluripotent stem cell (iPSC) generation has almost entirely been shaped by studies performed on reprogramming fibroblasts. However, whether the resulting model universally applies to the reprogramming process of other cell types is still largely unknown. By characterizing and profiling the reprogramming pathways of fibroblasts, neutrophils, and keratinocytes, we unveil that key events of the process, including loss of original cell identity, mesenchymal to epithelial transition, the extent of developmental reversion, and reactivation of the pluripotency network, are to a large degree cell-type specific. Thus, we reveal limitations for the use of fibroblasts as a universal model for the study of the reprogramming process and provide crucial insights about iPSC generation from alternative cell sources.