Effects of Brucella abortus biotype 1 infection on the reproductive performance of Sprague-Dawley rats.

The aim of this study was to assess the impact of Brucella abortus biotype 1 infection on the reproductive performance using Sprague-Dawley (SD) rat model. Virgin female SD rats (n = 24) were infected intraperitoneally with 0.1 mL of saline containing 1 x 10(11) Colony Forming Unit (CFU) of pathogenic B. abortus biotype 1 Korean bovine isolate. Control rats (n = 24) were inoculated with 0.1 mL of apyrogenic saline. Both inoculated and control rats were divided into six subgroups. Four rats in each subgroup were consistently bred at 3, 7, 14, 21, 28 and 60 days after infection. B. abortus infection induced 41.67% infertility in the infected rats. The mean number of offspring/litter was 8.71 +/- 2.01 for infected rats and 12.87 +/- 1.42 for control rats (p < 0.001). The mean weight of the viable offspring was 6.10 +/- 0.36 g for infected rats and 7.15 +/- 0.40 g for control rats (p < 0.001). The rate of stillbirth was 12.30% in the infected rats. B. abortus biotype 1 was isolated from the uteri of the infected rats. The data of this study indicate that B. abortus biotype 1 infections in SD rat model affect reproduction adversely by causing infertility, stillbirth and loss of number and weight of offspring.