Acute ankle sprain in a mouse model.

INTRODUCTION

Ankle sprains remain the most common orthopedic pathology. Conducting long-term studies in humans is difficult and costly, so the long-term consequences of an ankle sprain are not entirely known.

PURPOSE: The objective of this study is to develop and test a mechanical ankle instability model in mice.

METHODS

Thirty male mice (CBA/2J) were randomly placed into one of three groups: the transected calcaneal fibular ligament (CFL) group, the transected anterior talofibular ligament (ATFL)/CFL group, and a SHAM group. Three days after surgery, all of the mice were individually housed in a cage containing a solid surface running wheel, and daily running wheel measurements were recorded. Before and after surgery, measures of balance and gait were measured on all mice for 4 wk.

RESULTS

The mice in the ATFL/CFL group had significantly decreased duration (P = 0.0239), distance (P = 0.013), and speed (P = 0.003) compared with the SHAM group during week 1. During weeks 2 and 3, the ATFL/CFL group had significantly less distance (P = 0.0001) and duration (P = 0.002) compared with the SHAM and CFL-only group. The transection of the lateral ankle ligaments did affect the number of slips experienced during the balance test. The ATFL/CFL group had greater slips at 1 and 4 wk postsurgery (P = 0.05), whereas the CFL-only group had greater slips at 3 d and 1 wk postsurgery (P = 0.05). Relative to the SHAM group, the ATFL/CFL group and CFL-only group had smaller right-stride lengths (involved limb) at 3 d postsurgery (P = 0.05). The ATFL/CFL group also had smaller right-side stride lengths at 1 and 4 wk postsurgery (P = 0.05).

CONCLUSIONS

The results of this study indicate that a mouse model can be used to induce mechanical instability in the ankle.