The Journal of pharmacy and pharmacology · 2016
Cardioprotective effect of Salvianolic acid B on acute myocardial infarction by promoting autophagy and neovascularization and inhibiting apoptosis.
СтатьяНазвание и аннотация: Перевод в очереди
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Lin Chao, Liu Zhaoguo, Lu Ying, Yao Yuan, Zhang Yayun, Ma Zhi, Kuai Meiyu, Sun Xin, Sun Shuaijun, Jing Yi, Yu Lizhen, Li Yu, Zhang Qichun, Bian Huimin
Аннотация
OBJECTIVES: The aim of this study was to investigate the cardioprotective effect of salvianolic acid B (Sal B) on acute myocardial infarction (AMI) in rats and its potential mechanisms.
METHODS: The AMI model was established in rats to study the effect of Sal B on AMI. Haematoxylin-eosin (HE) staining was used to evaluate the pathological change in AMI rats. Immunofluorescence and TUNEL staining were used to detect autophagy and apoptosis of myocardial cells in hearts of AMI rats, respectively. Protein expression of apoptosis-related, autophagy-related and angiogenesis-related proteins were examined by Western blot.
KEY FINDINGS: Sal B attenuated myocardial infarction significantly compared with that of the model group. Rats administered with Sal B showed higher inhibition rate of infarction and lower infarct size than those of the model group. Moreover, Sal B decreased the serum levels of creatine kinase, lactate dehydrogenase and malondialdehyde, while increased such level of superoxide dismutase significantly compared with those of the model group. Sal B inhibited the expression of Bax, cleaved caspase-9 and cleaved PARP, while promoted the expression of Bcl-2, LC3-II, Beclin1 and VEGF.
CONCLUSIONS: Sal B has cardioprotective effect on AMI and Sal B may be a promising candidate for AMI treatment.
METHODS: The AMI model was established in rats to study the effect of Sal B on AMI. Haematoxylin-eosin (HE) staining was used to evaluate the pathological change in AMI rats. Immunofluorescence and TUNEL staining were used to detect autophagy and apoptosis of myocardial cells in hearts of AMI rats, respectively. Protein expression of apoptosis-related, autophagy-related and angiogenesis-related proteins were examined by Western blot.
KEY FINDINGS: Sal B attenuated myocardial infarction significantly compared with that of the model group. Rats administered with Sal B showed higher inhibition rate of infarction and lower infarct size than those of the model group. Moreover, Sal B decreased the serum levels of creatine kinase, lactate dehydrogenase and malondialdehyde, while increased such level of superoxide dismutase significantly compared with those of the model group. Sal B inhibited the expression of Bax, cleaved caspase-9 and cleaved PARP, while promoted the expression of Bcl-2, LC3-II, Beclin1 and VEGF.
CONCLUSIONS: Sal B has cardioprotective effect on AMI and Sal B may be a promising candidate for AMI treatment.
Предметные рубрики MeSH
Angiogenesis Inducing AgentsAnimalsApoptosisApoptosis Regulatory ProteinsAutophagyBeclin-1BenzofuransCardiotonic AgentsCreatine KinaseL-Lactate DehydrogenaseMaleMalondialdehydeMicrotubule-Associated ProteinsMyocardial InfarctionMyocardiumNeovascularization, PhysiologicRatsSuperoxide DismutaseVascular Endothelial Growth Factor ADepsides