The effect of IL-6/Piezo2 on the trigeminal neuropathic pain.

INTRODUCTION AND

OBJECTIVE

The impact of physical activity on human health is invaluable. Studies confirm an increase in neurotrophins in response to locomotor training. The Bcl-2 family of proteins, due to their diversity and pleiotropic actions, places damaged nerve cells on the path to apoptosis, making them a promising prospect in the treatment of neurodegenerative diseases. The aim of the experiment was to demonstrate the effect of the absence of the bcl-2 gene on the process of peripheral nerve regeneration in animals subjected to locomotor training.

MATERIAL AND

METHODS

The first group consisted of animals with an absent bcl-2 gene, 129S1/SvImJBcl2tm1Mpin/J (N=40). Group E consisted of animals subjected to sciatic nerve damage with absent bcl-2 gene (N=20), which were subjected to locomotor training, while mice in which the sciatic nerve was cut (N=20) but no training programme was applied represent group K. The evaluation used an automatic gait analysis system for mice and rats from Nodus system Cat&Walk. The study analyzed footprints on a treadmill controlling the mechanism of locomotor movements for analysis before sciatic nerve damage and at 7, 14 and 28 days after treatment.

RESULTS

Both strains showed statistically significant differences in the experimental group compared to the untrained group, as well as a statistically significant difference in trained mice between the Bcl-2 and bl6 strains at day 14 after sciatic nerve injury, and at day 28 after sciatic nerve injury in the Bcl-2 group compared to the bl6 group, both subjected to training.

CONCLUSIONS

Regeneration of the sciatic nerve in the locomotor-trained animals studied on the basis of the Cat&Walk study showed that significant improvement occurred in the group of Bcl-2 mice subjected to locomotor training on day 14 after nerve injury.