Open veterinary journal · 2026
Molecular characterization of CD59 and gmfb genes and their differential expression in Piaractus orinoquensis exposed to different conditions of brain injury.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Castro-Vargas Rafael Enrique, Herrera-Sánchez María Paula, Rondón-Barragán Iang
Аннотация
BACKGROUND: Neuroinflammation can be triggered by traumatic brain injury (TBI) and exposure to toxicants such as chlorpyrifos (CPF), leading to chronic disabilities and substantial health care costs. CD59 and glial maturation factor-beta proteins are implicated in various brain regulatory mechanisms. Investigating their roles can provide valuable insights into their role in pathologies such as TBI.
AIM: This study aimed to analyze the molecular characteristics of the CD59 and gmfb genes and assess their relative gene expression in brain tissues of Piaractus orinoquensis subjected to brain injury using quantitative PCR (qPCR).
METHODS: Thirty P. orinoquensis species were allocated to brain injury or CFP exposure models. Brain injury was induced by telencephalic stab puncture, and CPF exposure was administered at a sublethal concentration of 0.011 µg/l. Brain regions, including the olfactory bulb, telencephalon, and optic chiasm, were collected at designated time points, and qPCR analysis was performed. CD59 and gmfb genes were sequenced and characterized using bioinformatic tools.
RESULTS: Both genes exhibited conserved features compared with other fish species. In the brain injury model, fish subjected to brain injury showed reduced expression of CD59 and gmfb compared with controls, indicating a loss of immunomodulation associated with brain damage. Conversely, in the CPF-treated group, increased expression of CD59 in the olfactory bulb and gmfb in the optic chiasm was observed.
CONCLUSION: Downregulation of CD59 after brain injury suggests a loss of immunomodulatory capacity following brain damage. In CPF-exposed fish, the overexpression of both CD59 and gmfb in some brain tissues demonstrated the toxicant's ability to generate region-specific immunoactivity and neurotoxicity in the brain.
AIM: This study aimed to analyze the molecular characteristics of the CD59 and gmfb genes and assess their relative gene expression in brain tissues of Piaractus orinoquensis subjected to brain injury using quantitative PCR (qPCR).
METHODS: Thirty P. orinoquensis species were allocated to brain injury or CFP exposure models. Brain injury was induced by telencephalic stab puncture, and CPF exposure was administered at a sublethal concentration of 0.011 µg/l. Brain regions, including the olfactory bulb, telencephalon, and optic chiasm, were collected at designated time points, and qPCR analysis was performed. CD59 and gmfb genes were sequenced and characterized using bioinformatic tools.
RESULTS: Both genes exhibited conserved features compared with other fish species. In the brain injury model, fish subjected to brain injury showed reduced expression of CD59 and gmfb compared with controls, indicating a loss of immunomodulation associated with brain damage. Conversely, in the CPF-treated group, increased expression of CD59 in the olfactory bulb and gmfb in the optic chiasm was observed.
CONCLUSION: Downregulation of CD59 after brain injury suggests a loss of immunomodulatory capacity following brain damage. In CPF-exposed fish, the overexpression of both CD59 and gmfb in some brain tissues demonstrated the toxicant's ability to generate region-specific immunoactivity and neurotoxicity in the brain.