Wei sheng yan jiu = Journal of hygiene research · 2025
[Protective effects of 5-n-alkylresorcinols on H_2O_2-induced oxidative damage in Caco-2 cells].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Fan Zhixuan, Gong Zhaolong, Chen Chen, Wang Jingbo, Wang Liyuan, Qin Wen, Zhuo Qin, Luan Jing, Yang Zhuo
Аннотация
OBJECTIVE: To investigate the protective effects of five alkylresorcinol homologs on hydrogen peroxide(H_2O_2)-induced oxidative damage in human colorectal adenocarcinoma cells(Caco-2).
METHODS: The H_2O_2-induced oxidative damage of Caco-2 cells was used as a model, and the survival rate of Caco-2 cells pretreated with different concentrations(0-80 μmol/L)of 5-n-alkylresorcinols was determined to determine the optimal concentration range. The protective effects of 5-n-alkylresorcinols on oxidative-damaged cells was evaluated by measuring the levels of reactive oxygen species(ROS), malondialdehyde(MDA), glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) and catalase(CAT) activities.
RESULTS: 5-n-alkylresorcinols had no obvious toxic effect on Caco-2 cells in the concentration range of 5-80 μmol/L. The activities of GSH-Px, SOD and CAT oxidase in cell culture supernatant and cells were significantly lower than those in the control group after 4 hours of 600 μmol/L H_2O_(2 )treatment(P<0.05). The addition of 5 alkylresorcinol homologues at different concentrations alleviated the damage of H_2O_2 to different degrees, and the ROS level and MDA content decreased compared with the modularity, and the activity of GSH-Px, SOD and CAT was restored to a certain extent.
CONCLUSION: 5-n-alkylresorcinols can protect Caco-2 cells from oxidative damage to H_2O_2 by activating intracellular antioxidant enzyme activity and inhibiting lipid peroxidation levels.
METHODS: The H_2O_2-induced oxidative damage of Caco-2 cells was used as a model, and the survival rate of Caco-2 cells pretreated with different concentrations(0-80 μmol/L)of 5-n-alkylresorcinols was determined to determine the optimal concentration range. The protective effects of 5-n-alkylresorcinols on oxidative-damaged cells was evaluated by measuring the levels of reactive oxygen species(ROS), malondialdehyde(MDA), glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) and catalase(CAT) activities.
RESULTS: 5-n-alkylresorcinols had no obvious toxic effect on Caco-2 cells in the concentration range of 5-80 μmol/L. The activities of GSH-Px, SOD and CAT oxidase in cell culture supernatant and cells were significantly lower than those in the control group after 4 hours of 600 μmol/L H_2O_(2 )treatment(P<0.05). The addition of 5 alkylresorcinol homologues at different concentrations alleviated the damage of H_2O_2 to different degrees, and the ROS level and MDA content decreased compared with the modularity, and the activity of GSH-Px, SOD and CAT was restored to a certain extent.
CONCLUSION: 5-n-alkylresorcinols can protect Caco-2 cells from oxidative damage to H_2O_2 by activating intracellular antioxidant enzyme activity and inhibiting lipid peroxidation levels.