Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2026
Bio-Nanoparticle-Induced Self-Defense in Arabidopsis thaliana: Molecular Insights into Resistance Gene Activation Under Smart Industrial Stimuli.
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Ahmed Rouya Mohammed, Ali Hiba Naser, Majeed Adian Khalid, Alyasiri Thura, Amshawee Ahmed M, Hussain Maryam A, Hasan Ahmed Flayyih, El-Wahsh Hany M
Аннотация
BACKGROUND/AIMS: Nanobiotechnology offers sustainable strategies to enhance plant resistance by activating innate immune responses. This study evaluates the effect of chitosan nanoparticles (CNPs) on transcriptional activation of defense-associated genes in Arabidopsis thaliana.
METHODS: CNPs were produced via ionic gelation and sprayed on leaves of 4-week-old plants. Relative expression levels of PR1, PDF1.2, and WRKY70 were measured using qRT-PCR and the 2^-ΔΔCt method with normalization and statistical analysis.
RESULTS: CNP treatment significantly increased PR1, PDF1.2, and WRKY70 expression compared to controls, indicating activation of salicylic acid and jasmonic acid/ethylene signaling pathways. WRKY70 showed the highest induction, suggesting a major regulatory role in coordinating defense signaling.
CONCLUSION: Chitosan nanoparticles act as effective inducers of transcriptional activation of plant defense markers in Arabidopsis thaliana. Mechanisms including ROS signaling, calcium influx, MAPK cascades, and PTI/ETI responses warrant further investigation.
METHODS: CNPs were produced via ionic gelation and sprayed on leaves of 4-week-old plants. Relative expression levels of PR1, PDF1.2, and WRKY70 were measured using qRT-PCR and the 2^-ΔΔCt method with normalization and statistical analysis.
RESULTS: CNP treatment significantly increased PR1, PDF1.2, and WRKY70 expression compared to controls, indicating activation of salicylic acid and jasmonic acid/ethylene signaling pathways. WRKY70 showed the highest induction, suggesting a major regulatory role in coordinating defense signaling.
CONCLUSION: Chitosan nanoparticles act as effective inducers of transcriptional activation of plant defense markers in Arabidopsis thaliana. Mechanisms including ROS signaling, calcium influx, MAPK cascades, and PTI/ETI responses warrant further investigation.