The Hoverfly Attracting Property of a Methyl Salicylate-Containing (E)-β-Farnesene Analog (3e) and Potential Mechanism by Mediating the EcorOBP15 and EcorOR3.

Using natural enemies provides a sustainable method to control major agricultural pests. Hoverflies are significant natural enemies of aphids and efficient pollinators. Herbivore-induced plant volatiles (HIPVs), including (E)-β-farnesene (EBF) and methyl salicylate (MeSA), are key olfactory cues mediating hoverflies behavior. Our previous work identified compound 3e, an EBF analog containing a MeSA moiety, exhibited aphid-repelling and ladybug-attracting activities. However, whether 3e can attract hoverflies remains unknown. Therefore, this study explored the attractant property and potential mechanism of 3e toward hoverflies. Laboratory bioassays and field trials indicated 3e has an obvious hoverfly attracting property. The attraction mechanism studies demonstrate that, similar to EBF, 3e can interact with EcorOBP15 and EcorOR3, with its greater chemical softness, larger hydrophobic and charge regions enhancing these interactions. Furthermore, 3e exhibited low toxicity to honeybees (Apis mellifera) and hoverflies (Eupeodes corollae). Consequently, 3e could be a promising eco-friendly behavioral regulator for integrated aphid management in sustainable agriculture.