[The Role of Iron Dysregulation in White Matter Injury Induced by Carbon Mono- xide Poisoning].

OBJECTIVES

To explore the possible mechanisms of changes in neural metabolic cells in the white matter injury sites of the brain in mice with carbon monoxide (CO) poisoning and the abnormal absorption of iron ions in the body caused by intestinal flora and intestinal flora disorders.

METHODS

C57BL/6 mice were placed in a closed jar and given CO to establish a mouse model of CO poisoning. The control group was given the same treatment but not given CO. Samples were taken from the white matter to observe the morphological changes of white matter, the expression of transferrin (TRF) and glutathione peroxidase 4 (GPX4) was analyzed by immunohistochemistry and Western blotting. Meanwhile, feces of mice were collected at 3rd and 7th day after CO exposure, and 16S rRNA sequencing was performed to analyze the changes of intestinal flora in mice caused by CO poisoning.

RESULTS

Electron microscopy showed that CO poisoning caused obvious demyelination, with increased expression of TRF and decreased expression of GPX4 in the white matter (P<0.05). CO poisoning led to abnormal distribution of intestinal flora in mice, resulting in abnormal absorption of iron in intestinal tissue.

CONCLUSIONS

CO poisoning can cause damage to white matter nerve cells in the brain, leading to demyelination. It can also cause abnormal intestinal flora distribution and iron absorption in mice.