Inhibition by brimonidine of forskolin-induced nitrite production in isolated pig ciliary processes.

PURPOSE: To investigate by which mechanism the ocular hypotensive drug brimonidine (selective alpha(2)-adrenoreceptor agonist) inhibits the production of nitrite induced by forskolin in isolated porcine ciliary processes.

METHODS

Nitrite (a nitric oxide metabolite) was measured by Griess reaction in the medium surrounding the ciliary processes, before and after exposure to different drugs. Tissues were exposed for 120 minutes to forskolin (0.1 microM; an adenylylcyclase activator) or 8-bromo-cAMP (10 microM; a cAMP analogue). Some experiments were conducted in the presence of brimonidine (0.01-10 microM), yohimbine (0.1-10 microM; alpha(2)-adrenoreceptor antagonist), prazosin (10 microM; alpha(1)-adrenoreceptor antagonist), nicergoline (10 microM; an alpha(1)-adrenoreceptor antagonist), propranolol (10 microM; a beta-adrenoreceptor antagonist or beta-blocker), and/or pertussis toxin (2 microg/mL; PTX, a G(i)-protein inhibitor).

RESULTS

Nitrite production induced by forskolin (133% +/- 6%), but not that induced by 8-bromo-cAMP (133% +/- 6%), was inhibited in a concentration-dependent manner by brimonidine (10 microM: 103% +/- 4%, P < 0.001; EC(50): 0.05 microM). The inhibitory effect of brimonidine was prevented by PTX (119% +/- 7%, P < 0.01) and, in a concentration-dependent manner, by yohimbine (10 microM: 134% +/- 9%; P < 0.01), but not by prazosin, nicergoline, or propranolol.

CONCLUSIONS

Reduction of the formation of aqueous humor in the ciliary body's epithelium (and thus an intraocular pressure decrease) after alpha(2)-adrenergic receptor stimulation by brimonidine is known to be associated with a G(i)-protein-mediated inhibition of adenylylcyclase activity. The present study indicates that, through a similar alpha(2)-adrenoreceptor/G(i)-protein pathway, brimonidine can also inhibit nitrite production after adenylylcyclase activation (forskolin-induced) in isolated porcine ciliary processes.