Curcumin mitigates gas explosion-induced traumatic brain injury in male rats by regulating key molecular signatures: A multiomics analysis.

Treatment with curcumin following gas explosion-induced traumatic brain injury (TBI) can mitigate inflammatory brain damage; however, the underlying mechanisms remain unclear. This study performed transcriptomic and proteomic analyses of brain tissue from a rat GE-induced TBI model to identify related dysregulated molecules and signaling pathways. Additionally, an integrated transcriptomic and proteomic analysis was performed, and key molecular signatures were validated in blood serum and brain tissue. Treatment with curcumin significantly reduced histological abnormalities and microscopic structural changes induced by the gas explosion trauma in the brain. Further, it markedly reduced gas explosion-induced the levels of neuroinflammatory signaling molecules, including interleukin-1β and TNF-α. Notably, curcumin modulated 547 differentially expressed genes, which were primarily associated with the cAMP signaling pathway. This study identified candidate molecules and their perturbed biological pathways in gas explosion-induced TBI and explored the effects of curcumin using multi-omics approaches. SIGNIFICANCE: There remains insufficient understanding of gas explosion-induced TBI, and no relevant studies have been performed using multiple omics analysis to clarify the mechanisms of gas exposure-induced TBI in male rats. This important aspect of the unknown need to be further studied and explored. This study provides a biologically grounded, multi-omics characterization of gas explosion-induced traumatic brain injury by integrating quantitative proteomics with transcriptomic profiling in a controlled animal model. By identifying convergent dysregulation of inflammatory and cAMP-related signaling pathways and validating key molecular signatures at both the tissue and circulating levels, this study moves beyond descriptive proteomics toward pathway-level interpretation linked to structural and functional brain injury. The findings demonstrate how integrated proteomic analyses can be used to uncover mechanistic targets of neuroinflammation and therapeutic modulation, thereby contributing to the development of predictive biological models for blast-related brain injury and advancing the application of hypothesis-driven proteomics in neurotrauma research. This study focuses on curcumin treatment on the brain injury effects induced by gas explosion and stays in close touch with progress at the forefront of methods in modelling and simulation to aid mechanistic insights into neuroinflammation, immunoregulatory response, novel findings of therapeutic relevance to the central nervous system, and address modes of action including multiomics, systems biology, quantitative measurements biomarkers, histopathology, etc. So, we think it is appropriate for this journal.