International immunopharmacology · 2024
Berberine inhibits LPS-induced epithelial-mesenchymal transformation by activating the Nrf2 signalling pathway in bovine endometrial epithelial cells.
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Guo Yue, Zheng Zhikang, Zhang Guoxing, Zhong Jiaxing, Fan Xiucheng, Li Chunying, Zhu Shiyong, Cao Rongfeng, Fu Kaiqiang
Аннотация
BACKGROUND: Gram-negative bacteria are the primary pathogens of endometritis in dairy cows. LPS, the primary pathogenic agent of gram-negative bacteria, triggers an ROS increase, ultimately causing epithelial-mesenchymal transformation (EMT). Its significance in endometritis pathogenesis in dairy cows cannot be overlooked.
PURPOSE: Our previous studies showed that berberine could activate the Nrf2 signalling pathway, but whether it can inhibit the effect of LPS-induced EMT is uncharacterized.
METHODS: This research examined how berberine protects bovine endometrial epithelial cells (BEECs) by treating them with the compound for 2 h before exposing them to LPS to induce injury. Subsequently, the levels of reactive oxygen species (ROS) and epithelial-mesenchymal transition (EMT) markers in BEECs were quantified using the DCFH-DA probe, RT-qPCR, and Western blotting techniques.
RESULTS: Our investigation revealed that the triggering of the Nrf2 signal transduction pathway can effectively prevent LPS-induced EMT by reducing ROS levels in BEECs. Additionally, we found that berberine inhibits LPS-induced EMT by activating Nrf2 to reduce ROS levels.
CONCLUSION: These results suggest that berberine reduces ROS levels by upregulating the Nrf2 pathway in BEECs stimulated with LPS.
PURPOSE: Our previous studies showed that berberine could activate the Nrf2 signalling pathway, but whether it can inhibit the effect of LPS-induced EMT is uncharacterized.
METHODS: This research examined how berberine protects bovine endometrial epithelial cells (BEECs) by treating them with the compound for 2 h before exposing them to LPS to induce injury. Subsequently, the levels of reactive oxygen species (ROS) and epithelial-mesenchymal transition (EMT) markers in BEECs were quantified using the DCFH-DA probe, RT-qPCR, and Western blotting techniques.
RESULTS: Our investigation revealed that the triggering of the Nrf2 signal transduction pathway can effectively prevent LPS-induced EMT by reducing ROS levels in BEECs. Additionally, we found that berberine inhibits LPS-induced EMT by activating Nrf2 to reduce ROS levels.
CONCLUSION: These results suggest that berberine reduces ROS levels by upregulating the Nrf2 pathway in BEECs stimulated with LPS.