A differentiated nasal epithelial cell model derived from children with acute wheeze and asthma.

BACKGROUND

The airway epithelium is the primary structural and functional airway barrier and orchestrates innate immunity. Some children may have underlying epithelial vulnerabilities that contribute to the pathogenesis of acute wheeze and asthma (AWA).

OBJECTIVE

To investigate the pediatric AWA epithelial environment and responses at time of exacerbation to elucidate cellular vulnerabilities; thus, a differentiated in vitro model with differentiated cell types is required.

METHODS

Here, feasibility of establishing a differentiated model of primary nasal epithelial cells from children presenting to hospital with AWA was assessed. This was followed by examination of mucociliary differentiation, barrier integrity and function, viral receptor expression, and appropriate pro-inflammatory and antiviral cytokines compared with nasal epithelial cells derived from children who were non-wheezing non-atopic and non-wheezing with positive atopy.

RESULTS

A differentiated pediatric AWA model was established with interparticipant heterogeneity reflected appropriately. The AWA model was of epithelial lineage and had mucociliary differentiation, barrier integrity and function, viral receptor expression, and the production of innate immune cytokines. However, the AWA epithelium had a more permeable paracellular barrier, higher expression of rhinovirus receptor ICAM1, intrinsically higher levels of pro-inflammatory and antiviral cytokines IL-6, IL-8, CCL5, CXCL10, and IFNL1, and potentially fewer cilia. Relationships between demographic and clinical data and AWA epithelial parameters were also identified.

CONCLUSION

Collectively, a differentiated epithelial model of pediatric AWA was established, and results indicated underlying epithelial vulnerabilities in these children. This platform may be used to investigate the role of the AWA epithelium in pathogen infections, disease etiology, and therapeutic pipelines.