Riverine songbirds capture high levels of atmospheric mercury pollution from brown food webs in forests by mercury isotopic evidence.

Elevated methylmercury (MeHg) exposure poses significant risks to bird health, behavior, and reproduction. Still, the risk of MeHg exposure to forest birds, accounting for over 80 % of the world's bird species, is poorly understood. This study combines Hg isotopes and video analysis, aiming to assess MeHg exposure risks to a forest riverine songbird, the spotted forktail (Enicurus maculatus) from a remote subtropical montane forest. Noticeably, 83 % of feather MeHg concentrations of adult forktails exceeded 5000 ng g-1, a threshold level potentially impacting bird reproduction, and 50 % of feather MeHg concentrations in forktail nestlings exceeded the threshold level of 1000 ng g-1, that potentially impacts the nestling growth. Forktail nestlings ingested ∼ 99 % of their MeHg from prey within brown food webs (i.e., from forest floor, aquatic, and emergent aquatic prey). The Hg isotopes reveal that MeHg along the bird food chain is mostly derived from in situ methylation of litterfall deposited atmospheric Hg0, with limited photo-demethylation (i.e., 4-12 %) in shaded forest environments. The risk of MeHg exposure of forest songbirds correlated positively with the proportion of prey consumed from brown food webs. We recommend incorporating resident riverine songbirds in monitoring programs to better evaluate the effectiveness of the Minamata Convention, especially in remote forest ecosystems where in situ MeHg production may be underestimated.