Life sciences · 2013
Luteolin attenuates TGF-β1-induced epithelial-mesenchymal transition of lung cancer cells by interfering in the PI3K/Akt-NF-κB-Snail pathway.
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Chen Kun-Chieh, Chen Chiu-Yuan, Lin Chih-Ru, Yang Tsung-Ying, Chen Tzu-Hsiu, Wu Li-Chen, Wu Chun-Chi
Аннотация
AIMS: Luteolin is a natural flavonoid that possesses a variety of pharmacological activities, such as anti-inflammatory and anti-cancer abilities. Whether luteolin regulates the transformation ability of lung cancer cells remains unclear. The current study aims to uncover the effects and underlying mechanisms of luteolin in regulation of and epithelial-mesenchymal transition of lung cancer cells.
MAIN METHODS: The lung adenocarcinoma A549 cells were used in this experiment; the cells were pretreated with luteolin followed by administration with TGF-β1. The expression levels of various cadherin and related upstream regulatory modules were examined.
KEY FINDINGS: Pretreatment of luteolin prevented the morphological change and downregulation of E-cadherin of A549 cells induced by TGF-β1. In addition, the activation of PI3K-Akt-IκBa-NF-κB-Snail pathway which leads to the decline of E-cadherin induced by TGF-β1 was also attenuated under the pretreatment of luteolin.
SIGNIFICANCE: We provide the mechanisms about how luteolin attenuated the epithelial-mesenchymal transition of A549 lung cancer cells induced by TGF-β1. This finding will strengthen the anti-cancer effects of flavonoid compounds via the regulation of migration/invasion and EMT ability of various cancer cells.
MAIN METHODS: The lung adenocarcinoma A549 cells were used in this experiment; the cells were pretreated with luteolin followed by administration with TGF-β1. The expression levels of various cadherin and related upstream regulatory modules were examined.
KEY FINDINGS: Pretreatment of luteolin prevented the morphological change and downregulation of E-cadherin of A549 cells induced by TGF-β1. In addition, the activation of PI3K-Akt-IκBa-NF-κB-Snail pathway which leads to the decline of E-cadherin induced by TGF-β1 was also attenuated under the pretreatment of luteolin.
SIGNIFICANCE: We provide the mechanisms about how luteolin attenuated the epithelial-mesenchymal transition of A549 lung cancer cells induced by TGF-β1. This finding will strengthen the anti-cancer effects of flavonoid compounds via the regulation of migration/invasion and EMT ability of various cancer cells.
Предметные рубрики MeSH
AdenocarcinomaAdenocarcinoma of LungBlotting, WesternCell Line, TumorCell SurvivalCells, CulturedChromatin ImmunoprecipitationDNA-Binding ProteinsElectrophoretic Mobility Shift AssayEpithelial-Mesenchymal TransitionExpectorantsFluorescent Antibody Technique, IndirectGene Expression Regulation, NeoplasticHumansIndicators and ReagentsLung NeoplasmsLuteolinNF-kappa BOncogene Protein v-aktPhosphatidylinositol 3-KinasesReal-Time Polymerase Chain ReactionSignal TransductionSnail Family Transcription FactorsTranscription FactorsTransforming Growth Factor beta1