Nitric oxide and AMF-mediated regulation of soil enzymes activities, cysteine-H2S system and thiol metabolites in mitigating chromium (Cr (VI)) toxicity in pigeonpea genotypes.

Cr (VI) hampers plant growth and yield by reducing essential nutrient uptake as it competes for phosphate and sulfate transporters. Nitric oxide (NO) and mycorrhization play important roles in mitigating Cr (VI) toxicity. Present study aimed to compare the potential of AMF (Arbuscular mycorrhizal fungi)-Rhizoglomus intraradices and NO (0.25 mM) in alleviating Cr (VI) stress (0, 10 and 20 mg/kg) in two differentially tolerant pigeonpea genotypes (Pusa 2001 and AL 201). Cr (VI) toxicity reduced growth, mycorrhizal colonization, nutrient uptake, and overall productivity by inducing reactive oxygen species (ROS) generation, with AL 201 more sensitive than Pusa 2001. NO and AM enhanced activities of soil enzymes, thereby increasing nutrients availability as well as their uptake, with AM more effective than NO. Both amendments reduced oxidative stress and restricted Cr (VI) uptake by increasing the activities of antioxidant and S- assimilatory enzymes, with Pusa 2001 more responsive than AL 201. NO was relatively more efficient in regulating cysteine-H2S system by increasing the activities of biosynthetic enzymes (ATP-sulfurylase (ATPS), O-acetylserine thiol lyase (OASTL), D-cysteine desulfhydrase (DCD) and L-cysteine desulfhydrase (LCD), while AM significantly increased glutathione reductase (GR), γ-glutamylcysteine synthetase (γ-ECS) enzymes activities and resultant glutathione (GSH), phytochelatins (PCs), and non-protein thiols (NP-SH) synthesis. Moreover, co-application of NO and AM proved to be highly beneficial in negating the toxic effects of Cr (VI) due to functional complementarity between them. Study suggested the combined use of NO and AM as a useful strategy in re-establishing pigeonpea plants growing in Cr (VI)-stressed environments.