Diminished attention network activity and heightened salience-default mode transitions in generalized anxiety disorder: Evidence from resting-state EEG microstate analysis.

Generalized anxiety disorder (GAD) is a common anxiety disorder characterized by excessive, uncontrollable worry and physical symptoms such as difficulty concentrating and sleep disturbances. Although functional magnetic resonance imaging (fMRI) studies have reported aberrant network-level activity related to cognition and emotion in GAD, its low temporal resolution restricts its ability to capture the rapid neural activity in mental processes. EEG microstate analysis offers millisecond-resolution for tracking the dynamic changes in brain electrical activity, thereby illuminating the neurophysiological mechanisms underlying the cognitive and emotional dysfunctions in GAD. This study collected 64-channel resting-state EEG data from 28 GAD patients and 28 healthy controls (HC), identifying five microstate classes (A-E) in both groups. Results showed that GAD patients exhibited significantly lower duration (p < 0.01), occurrence (p < 0.05), and coverage (p < 0.01) of microstate class D, potentially reflecting deficits in attention-related networks. Such alterations may contribute to the impairments in attention maintenance and cognitive control. Additionally, GAD patients displayed reduced transition probabilities in A → D, B → D, C → D, and E → D (all corrected p < 0.05), but increased in C → E (corrected p < 0.05) and E → C (corrected p < 0.01). These results highlight a significant reduction in the brain's ability to transition into microstate class D, alongside overactivity in switching between the default mode network and the salience network. Such neurophysiological changes may underlie cognitive control deficits, increased spontaneous rumination, and emotional regulation challenges observed in GAD. Together, these insights provide a new perspective for understanding the neurophysiological and pathological mechanisms underlying GAD.