Nature communications · 2018
Intraamniotic Zika virus inoculation of pregnant rhesus macaques produces fetal neurologic disease.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Coffey Lark L, Keesler Rebekah I, Pesavento Patricia A, Woolard Kevin, Singapuri Anil, Watanabe Jennifer, Cruzen Christina, Christe Kari L, Usachenko Jodie, Yee JoAnn, Heng Victoria A, Bliss-Moreau Eliza, Reader J Rachel, von Morgenland Wilhelm, Gibbons Anne M, Jackson Kenneth, Ardeshir Amir, Heimsath Holly, Permar Sallie, Senthamaraikannan Paranthaman, Presicce Pietro, Kallapur Suhas G, Linnen Jeffrey M, Gao Kui, Orr Robert, MacGill Tracy, McClure Michelle, McFarland Richard, Morrison John H, Van Rompay Koen K A
Аннотация
Zika virus (ZIKV) infection of pregnant women can cause fetal microcephaly and other neurologic defects. We describe the development of a non-human primate model to better understand fetal pathogenesis. To reliably induce fetal infection at defined times, four pregnant rhesus macaques are inoculated intravenously and intraamniotically with ZIKV at gestational day (GD) 41, 50, 64, or 90, corresponding to first and second trimester of gestation. The GD41-inoculated animal, experiencing fetal death 7 days later, has high virus levels in fetal and placental tissues, implicating ZIKV as cause of death. The other three fetuses are carried to near term and euthanized; while none display gross microcephaly, all show ZIKV RNA in many tissues, especially in the brain, which exhibits calcifications and reduced neural precursor cells. Given that this model consistently recapitulates neurologic defects of human congenital Zika syndrome, it is highly relevant to unravel determinants of fetal neuropathogenesis and to explore interventions.