Consortia of Bacillus sp. LY05 and Bacillus cereus LGY06 immobilized on coconut shell charcoal remediates pendimethalin and cadmium contaminated sites in-situ and alleviates peanut's continuous cropping obstacles.

The use of artificial addition of microbial consortia into soils is a key strategy to simultaneously alleviate the common contamination and continuous cropping obstacles. This study established the coconut shell charcoal (CSC) LY05 +LGY06 by immobilizing Bacillus sp. LY05 and B. cereus LGY06 on CSC particles, which effectively accelerated the removal of pendimethalin as a possible human carcinogen (91.68 %) and Cd2 + (80.02 %) from the culture-solution, and displayed the prominent re-usability and stability compared with their free coculture consortium. This consortium could detoxify pendimethalin via serial nitro-reduction, oxidation, cyclization, carboxylation and hydroxylation reactions. CSC LY05 +LGY06 could rapidly degrade pendimethalin in sterile (90.01 %) and non-sterile (99.03 %) soils. Moreover, it notably reduced pendimethalin and Cd2+ residuals in pod kernels, pod shells, and plants of peanut growing in continuous cropping soil by 99.19 % and 94.02 %, 99.16 % and 93.94 %, and 98.94 % and 91.85 %, respectively, contrasted to the control. Meanwhile, it significantly promoted the pod yield (>1.28-fold) and quality of continuous cropping peanut by improving the plant agronomic trait, photosynthetic capacity, intracellular water metabolism, nutrient transport capacity, and metabolic activity. This study provides a novel insight into the safe production of agri-products in continuous cropping soils con-contaminated by pesticides and heavy metals.