Epigenomics · 2016
Tagging methyl-CpG-binding domain proteins reveals different spatiotemporal expression and supports distinct functions.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Wood Kathleen H, Johnson Brian S, Welsh Sarah A, Lee Jun Y, Cui Yue, Krizman Elizabeth, Brodkin Edward S, Blendy Julie A, Robinson Michael B, Bartolomei Marisa S, Zhou Zhaolan
Аннотация
AIM: DNA methylation is recognized by methyl-CpG-binding domain (MBD) proteins. Multiple MBDs are linked to neurodevelopmental disorders in humans and mice. However, the functions of MBD2 are poorly understood. We characterized Mbd2 knockout mice and determined spatiotemporal expression of MBDs and MBD2-NuRD (nucleosome remodeling deacetylase) interactions.
EXPERIMENTAL PROCEDURES: We analyzed behavioral phenotypes, generated biotin-tagged MBD1 and MBD2 knockin mice, and performed biochemical studies of MBD2-NuRD.
RESULTS: Most behavioral measures are minimally affected in Mbd2 knockout mice. In contrast to other MBDs, MBD2 shows distinct expression patterns.
CONCLUSION: Unlike most MBDs, MBD2 is ubiquitously expressed in all tissues examined and appears dispensable for brain functions measured in this study. We provide novel genetic tools and reveal new directions to investigate MBD2 functions in vivo.
EXPERIMENTAL PROCEDURES: We analyzed behavioral phenotypes, generated biotin-tagged MBD1 and MBD2 knockin mice, and performed biochemical studies of MBD2-NuRD.
RESULTS: Most behavioral measures are minimally affected in Mbd2 knockout mice. In contrast to other MBDs, MBD2 shows distinct expression patterns.
CONCLUSION: Unlike most MBDs, MBD2 is ubiquitously expressed in all tissues examined and appears dispensable for brain functions measured in this study. We provide novel genetic tools and reveal new directions to investigate MBD2 functions in vivo.
Предметные рубрики MeSH
AnimalsBehavior, AnimalBiogenic AminesBody WeightBrainCpG IslandsDNA MethylationDNA-Binding ProteinsFemaleGene Expression RegulationGene Knock-In TechniquesMaleMaze LearningMethyl-CpG-Binding Protein 2Mi-2 Nucleosome Remodeling and Deacetylase ComplexMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMotor ActivitySpatio-Temporal AnalysisTranscription Factors