Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2026
[Hypolipidemic activity of phenolic acids from Inula japonica Thunb].
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Chen Yuting, You Yanting, Huang Yushu, He Fei, Qiu Feng, DU Qingfeng
Аннотация
OBJECTIVES: To isolate and identify phenolic acid constituents from methanol extract of Inula japonica Thunb. and evaluate their lipid-lowering activity in vitro.
METHODS: The methanol extract of Inula japonica Thunb. was purified using silica gel, ODS, and semi-preparative HPLC. The compound structures were determined using physicochemical and spectroscopic analyses, and their hypolipidemic activities were assessed in an AML12 cell model of free fatty acids (FFA)-induced lipid deposition. The active compounds were further assessed for their effects on lipid deposition, reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) of the cell model.
RESULTS: A total of 11 phenolic acid compounds were isolated from Inula japonica Thunb., namely dihydrocaffeic acid (1), protocatechualdehyde (2), chlorogenic acid (3), cryptochlorogenic acid (4), 1,3-O-dicaffeoylquinic acid (5), 2,3-dicarboxy-6,7-hydroxy-l-(3',4'-dihydroxy)-phenyl -1,2-dihydronaphthalene-10-methyl ester (6), caffeic acid (7), protocatechuic acid (8), 3-carboxy-6,7-dihydroxy-1-(3',4'-dihydroxyphenyl)-naphthalene (9), p-hydroxybenzoic acid (10), and 4-hydroxyphenylacetic acid (11). Among them, compounds 1 and 9 were isolated for the first time from the genus Inula, and compound 6 was isolated for the first time from this plant. Compounds 6 and 9 showed lipid-lowering effects in FFA-induced AML12 cells, significantly lowered cellular levels of total cholesterol, triglyceride, alanine aminotransferase, and aspartate aminotransferase, reduced lipid accumulation, and alleviated oxidative stress and mitochondrial function.
CONCLUSIONS: Inula japonica Thunb. contains diverse phenolic acids, and among them compounds 6 and 9 show hypolipidemic activities via inhibiting lipid accumulation and improving oxidative stress and mitochondrial function and can potentially serve as lead compounds for treatment of metabolic dysfunction-associated steatotic liver disease.
METHODS: The methanol extract of Inula japonica Thunb. was purified using silica gel, ODS, and semi-preparative HPLC. The compound structures were determined using physicochemical and spectroscopic analyses, and their hypolipidemic activities were assessed in an AML12 cell model of free fatty acids (FFA)-induced lipid deposition. The active compounds were further assessed for their effects on lipid deposition, reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) of the cell model.
RESULTS: A total of 11 phenolic acid compounds were isolated from Inula japonica Thunb., namely dihydrocaffeic acid (1), protocatechualdehyde (2), chlorogenic acid (3), cryptochlorogenic acid (4), 1,3-O-dicaffeoylquinic acid (5), 2,3-dicarboxy-6,7-hydroxy-l-(3',4'-dihydroxy)-phenyl -1,2-dihydronaphthalene-10-methyl ester (6), caffeic acid (7), protocatechuic acid (8), 3-carboxy-6,7-dihydroxy-1-(3',4'-dihydroxyphenyl)-naphthalene (9), p-hydroxybenzoic acid (10), and 4-hydroxyphenylacetic acid (11). Among them, compounds 1 and 9 were isolated for the first time from the genus Inula, and compound 6 was isolated for the first time from this plant. Compounds 6 and 9 showed lipid-lowering effects in FFA-induced AML12 cells, significantly lowered cellular levels of total cholesterol, triglyceride, alanine aminotransferase, and aspartate aminotransferase, reduced lipid accumulation, and alleviated oxidative stress and mitochondrial function.
CONCLUSIONS: Inula japonica Thunb. contains diverse phenolic acids, and among them compounds 6 and 9 show hypolipidemic activities via inhibiting lipid accumulation and improving oxidative stress and mitochondrial function and can potentially serve as lead compounds for treatment of metabolic dysfunction-associated steatotic liver disease.