Phytochemical and biological investigations of Salvia microphylla leaf extracts using LC-MS/MS.

In this research, the total phenolics, antioxidant activity, and antibacterial susceptibility of Salvia microphylla leaf extracts were studied using solvents of varying polarity (n-hexane, dichloromethane, ethyl acetate, and 80% methanol). Total phenolic content (TPC) and total flavonoid content (TFC) were estimated using the Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. Phytochemical analysis was conducted using the LC-ESI-MS/MS method. By using the FRAP and DPPH methods, the antioxidant activities were measured spectrophotometrically. The antimicrobial assay was done through the agar-well diffusion method against the Gram-negative and Gram-positive bacteria. All extracts showed potent antimicrobial activity, with the hexane extract displaying strong inhibitory effects. These findings indicate that S. microphylla leaf extracts contain promising bioactive compounds possessing antioxidant and antibacterial properties, suggesting further research to isolate and characterise these bioactive compounds and assess their in vivo effects.