Construction of a chiral metal-organic framework material with triazole amino acid ligands and its application in capillary electrochromatography for enantioseparation.

Chiral separation is vital in medicine because the two versions of a chiral drug can act differently, and one might cause toxicity. Therefore, efficient enantioseparation is essential in pharmaceutical quality control. In this study, we modified the amino site of l-lysine (L-Lys) with a triazole ring. The triazole amino acid ligand (NL) with self-assembly potential was designed independently. Then NLMOF was synthesized by coordination with Cu (II) under green ambient stirring conditions. The NLMOF (triazole-modified metal-organic framework) was uniformly integrated into a capillary column through a post-modification method and utilized in open tubular capillary electrochromatography (OT-CEC) for enantioseparation. 10 chiral amino acids and 1 chiral drug was successfully separated in NLMOF@OT-CEC method with excellent repeatability (RSD < 5 %) and stability (cycles > 100). Furthermore, when compared with similar MOFs (metal-organic frameworks) chiral stationary phase, NLMOF demonstrates advantages in terms of mild preparation conditions, cost-effectiveness, and environmental sustainability, making it a promising candidate for industrial-scale production.