The fabrication and intelligent evaluation for meat freshness of colorimetric hydrogels using zein and sodium alginate loading anthocyanin and curcumin: Stability and sensitivity to pH and volatile amines.

Intelligent packaging designed to detect food spoilage is receiving increasing attention, and pH-sensitive colorimetric hydrogels show great potential for monitoring food spoilage. The pH-sensitive colorimetric hydrogels incorporating dual indicators of anthocyanin (BA) and curcumin (CUR) were fabricated via the interactions of zein and sodium alginate (SA) to assess meat freshness. The effects of the addition ratios of BA and CUR on zein/sodium alginate hydrogels were characterized by morphological observation, structural analysis and cumulative release profiles, and the sensitivity of the colorimetric hydrogels was also evaluated. The zein/sodium alginate hydrogel (zein/SA/Mix2), which incorporated the mixture of BA and CUR at a ratio of 70:1, exhibited the smallest particle size (1152.67 nm) and displayed a more homogeneous and dense gel structure compared with other treatments. FTIR and XRD results indicated that the interactions between sodium alginate and zein were primarily governed by hydrogen bonds and electrostatic forces, and the zein/SA/Mix2 hydrogel exhibited the weakest peak intensity at 3422 cm-1 and at 2θ = 28.25°, indicating the highest degree of crosslinking among these treatments. The zein/SA/Mix2 hydrogel rapidly responded to volatile amines within 2 min, and the release rates of BA and CUR remained below 26 % and 5 % in 95 % ethanol solution within 96 h, respectively, indicating its high stability and sensitivity. During the storage of air-dried goose meat and chilled chicken meat, the zein/SA/Mix2 hydrogel transitioned from yellow to green, and finally to dark brown, effectively distinguishing meat freshness, which was further confirmed by partial least squares regression analysis.