The roles of nesfatin-1 in the lateral parabrachial nucleus on feeding and glucose metabolism in type 1 diabetic rats.

Nesfatin-1 assumes a crucial role in the regulation of appetite and glucose metabolism. The present study aimed to determine the effect of LPBN nesfatin-1 on food intake and blood glucose in type 1 diabetes (T1DM) rats and underlying mechanisms. Catheter implantation and nucleus microinjection were employed to investigate the effect of LPBN nesfain-1 on the regulation of food intake and blood glucose in Rats with T1DM. In vivo electrophysiological technique was used to record LPBN glucose-sensitive (GS) neurons to explore central underlying mechanisms. ELISA kits were utilized to assess the plasma levels of nesfatin-1, norepinephrine (NE), and epinephrine (E). We found LPBN nesfatin-1 inhibited nocturnal feeding in Rats with T1DM. The glucose regulation of LPBN nesfatin-1 is closely associated with insulin. LPBN nesfatin-1 increased the firing rate of glucose-excited neurons in Rats with T1DM and the firing rate of glucose-inhibited neurons in Wistar Rats. LPBN nesfatin-1 increased plasma NE levels in Wistar Rats under hypoglycemia and decreased plasma E levels in Rats with T1DM with hyperglycemia. These findings suggested that LPBN nesfatin-1 was involved in food intake and blood glucose regulation in Rats with T1DM, which may be achieved by influencing GS neurons and subsequent putative modulation of sympathetic nervous system activity, a pathway that requires further mechanistic validation.