Fertility and sterility · 2007
Study of aneuploidy in large-headed, multiple-tailed spermatozoa: case report and review of the literature.
Клинический случайСтатьяПоддержка исследования: вне правительства СШАОбзорНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Perrin Aurore, Morel Frédéric, Moy Ludovic, Colleu Daniel, Amice Véronique, De Braekeleer Marc
Аннотация
OBJECTIVE: To determine the meiotic segregation in large-headed, multiple-tailed spermatozoa.
DESIGN: Analysis of sperm nuclei by fluorescence in situ hybridization (FISH).
SETTING: University hospital.
PATIENT(S): A 34-year-old man with 100% morphologically abnormal spermatozoa.
INTERVENTION(S): Dual-color FISH for chromosomes 13 and 21 and triple-color FISH for chromosomes X, Y, and 18 were performed.
MAIN OUTCOME MEASURE(S): Aneuploidy rates.
RESULT(S): More than 99% of the spermatozoa had abnormal content for chromosomes X, Y, 13, 18, and 21. Diploidy, triploidy, and tetraploidy rates were found to be 18.42%, 6.14%, and 33.99% in triple-color FISH and to be 16.09%, 16.28%, and 38.95% in dual-color FISH.
CONCLUSION(S): Our results and those from other investigators show that large-headed, multiple-tailed spermatozoa are associated with a high rate of polyploidy and aneuploidy. Intracytoplasmic sperm injection should not be recommended to those patients, not only because of its low success rate but also because of its high genetic risk.
DESIGN: Analysis of sperm nuclei by fluorescence in situ hybridization (FISH).
SETTING: University hospital.
PATIENT(S): A 34-year-old man with 100% morphologically abnormal spermatozoa.
INTERVENTION(S): Dual-color FISH for chromosomes 13 and 21 and triple-color FISH for chromosomes X, Y, and 18 were performed.
MAIN OUTCOME MEASURE(S): Aneuploidy rates.
RESULT(S): More than 99% of the spermatozoa had abnormal content for chromosomes X, Y, 13, 18, and 21. Diploidy, triploidy, and tetraploidy rates were found to be 18.42%, 6.14%, and 33.99% in triple-color FISH and to be 16.09%, 16.28%, and 38.95% in dual-color FISH.
CONCLUSION(S): Our results and those from other investigators show that large-headed, multiple-tailed spermatozoa are associated with a high rate of polyploidy and aneuploidy. Intracytoplasmic sperm injection should not be recommended to those patients, not only because of its low success rate but also because of its high genetic risk.