Frontiers in cellular and infection microbiology · 2026
Unusual case of Myroides odoratimimus infection in a cancer patient: addressing its antimicrobial resistance mechanisms and review of the literature.
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Fan Lingying, Chen Yiping
Аннотация
INTRODUCTION: Myroides odoratimimus is a Gram-negative opportunistic pathogen primarily affecting immunocompromised individuals. However, its resistance characteristics and therapeutic response in cancer patients remain poorly defined. This study investigated the antimicrobial resistance profile of a urinary M. odoratimimus isolate from a gastric cancer patient and evaluated the influence of environmental pH on antibiotic efficacy.
METHODS: The isolate was identified using the VITEK 2-Compact system, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 16S ribosomal ribonucleic acid (16S rRNA) sequencing. Antimicrobial susceptibility testing against 24 antibiotics was performed using the VITEK 2 system according to Clinical and Laboratory Standards Institute (CLSI) M100 (Ed33) guidelines. Resistance genes were detected by polymerase chain reaction (PCR) and sequencing. The impact of pH (5.5-8.5) on antibiotic activity was assessed using disk diffusion. In vivo efficacy of minocycline was evaluated in a murine urinary tract infection model with controlled urine pH modulation.
RESULTS: The isolate showed 100% sequence identity with M. odoratimimus PR63069 and exhibited multidrug resistance, remaining susceptible only to minocycline. Molecular analysis confirmed the presence of the β-lactamase gene (blaMUS-1). Tigecycline and minocycline demonstrated optimal activity under neutral conditions (pH 7.3), whereas piperacillin-tazobactam showed relatively improved activity at alkaline pH. In vivo, urinary alkalization (pH 8.5) significantly enhanced the antibacterial efficacy of minocycline.
CONCLUSION: M. odoratimimus isolated from a gastric cancer patient demonstrated multidrug resistance mediated by blaMUS-1. Antibiotic activity varied with pH, and urine alkalinization improved minocycline efficacy, suggesting potential strategies for clinical management of Myroides infections.
METHODS: The isolate was identified using the VITEK 2-Compact system, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 16S ribosomal ribonucleic acid (16S rRNA) sequencing. Antimicrobial susceptibility testing against 24 antibiotics was performed using the VITEK 2 system according to Clinical and Laboratory Standards Institute (CLSI) M100 (Ed33) guidelines. Resistance genes were detected by polymerase chain reaction (PCR) and sequencing. The impact of pH (5.5-8.5) on antibiotic activity was assessed using disk diffusion. In vivo efficacy of minocycline was evaluated in a murine urinary tract infection model with controlled urine pH modulation.
RESULTS: The isolate showed 100% sequence identity with M. odoratimimus PR63069 and exhibited multidrug resistance, remaining susceptible only to minocycline. Molecular analysis confirmed the presence of the β-lactamase gene (blaMUS-1). Tigecycline and minocycline demonstrated optimal activity under neutral conditions (pH 7.3), whereas piperacillin-tazobactam showed relatively improved activity at alkaline pH. In vivo, urinary alkalization (pH 8.5) significantly enhanced the antibacterial efficacy of minocycline.
CONCLUSION: M. odoratimimus isolated from a gastric cancer patient demonstrated multidrug resistance mediated by blaMUS-1. Antibiotic activity varied with pH, and urine alkalinization improved minocycline efficacy, suggesting potential strategies for clinical management of Myroides infections.
Предметные рубрики MeSH
AnimalsHumansMaleMiceAnti-Bacterial AgentsDisease Models, AnimalDNA, BacterialDNA, RibosomalDrug Resistance, BacterialFlavobacteriaceaeFlavobacteriaceae InfectionsHydrogen-Ion ConcentrationMicrobial Sensitivity TestsMinocyclineRNA, Ribosomal, 16SSequence Analysis, DNASpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStomach NeoplasmsUrinary Tract InfectionsMiddle Aged