Fertility and sterility · 2016
Characterization of the injection funnel during intracytoplasmic sperm injection reflects cytoplasmic maturity of the oocyte.
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Krause Iris, Pohler Uwe, Grosse Stefan, Shebl Omar, Petek Erwin, Chandra Arvind, Ebner Thomas
Аннотация
OBJECTIVE: To quantify cytoplasmic maturity on the basis of intracytoplasmic sperm injection (ICSI) injection funnel manifestation and to evaluate influence factors of the latter.
DESIGN: Prospective study.
SETTING: Private fertility center.
PATIENT(S): A total of 31 patients with good ovarian response.
INTERVENTION(S): Mature and immature oocytes were injected intracytoplasmatically. Formation and persistence of an injection funnel was documented and measured.
MAIN OUTCOME MEASURE(S): ICSI funnel size, persistence of injection funnel, rates of degeneration and fertilization, embryo quality.
RESULT(S): Funnel volume in germinal vesicle stage oocytes (prophase I [PI]) was significantly smaller than that of metaphase I (MI) and MII oocytes. Immature eggs (PI, MI) almost never showed a persistent funnel 2-4 minutes after ICSI, whereas in MII eggs the funnel was still observable in 35% (117/334) of the cases. Uni- and multivariate analysis revealed that pipette type and stimulation protocol significantly influenced appearance of injection funnel. Funnel volume in oocytes that fertilized regularly was significantly higher compared with three-polar body and degenerated oocytes.
CONCLUSION(S): Oocyte maturation within the follicle is closely associated with a remarkable change in cytoplasm viscosity from an aqueous to a more viscous subtype. Precise evaluation of the injection funnel may help to explain deviations from expected ICSI outcome and could also assist in optimizing controlled ovarian hyperstimulation.
DESIGN: Prospective study.
SETTING: Private fertility center.
PATIENT(S): A total of 31 patients with good ovarian response.
INTERVENTION(S): Mature and immature oocytes were injected intracytoplasmatically. Formation and persistence of an injection funnel was documented and measured.
MAIN OUTCOME MEASURE(S): ICSI funnel size, persistence of injection funnel, rates of degeneration and fertilization, embryo quality.
RESULT(S): Funnel volume in germinal vesicle stage oocytes (prophase I [PI]) was significantly smaller than that of metaphase I (MI) and MII oocytes. Immature eggs (PI, MI) almost never showed a persistent funnel 2-4 minutes after ICSI, whereas in MII eggs the funnel was still observable in 35% (117/334) of the cases. Uni- and multivariate analysis revealed that pipette type and stimulation protocol significantly influenced appearance of injection funnel. Funnel volume in oocytes that fertilized regularly was significantly higher compared with three-polar body and degenerated oocytes.
CONCLUSION(S): Oocyte maturation within the follicle is closely associated with a remarkable change in cytoplasm viscosity from an aqueous to a more viscous subtype. Precise evaluation of the injection funnel may help to explain deviations from expected ICSI outcome and could also assist in optimizing controlled ovarian hyperstimulation.