Cell reports · 2023
XLF/Cernunnos loss impairs mouse brain development by altering symmetric proliferative divisions of neural progenitors.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Bery Amandine, Etienne Olivier, Mouton Laura, Mokrani Sofiane, Granotier-Beckers Christine, Gauthier Laurent R, Feat-Vetel Justyne, Kortulewski Thierry, Pérès Elodie A, Desmaze Chantal, Lestaveal Philippe, Barroca Vilma, Laugeray Antony, Boumezbeur Fawzi, Abramovski Vincent, Mortaud Stéphane, Menuet Arnaud, Le Bihan Denis, Villartay Jean-Pierre de, Boussin François D
Аннотация
XLF/Cernunnos is a component of the ligation complex used in classical non-homologous end-joining (cNHEJ), a major DNA double-strand break (DSB) repair pathway. We report neurodevelopmental delays and significant behavioral alterations associated with microcephaly in Xlf-/- mice. This phenotype, reminiscent of clinical and neuropathologic features in humans deficient in cNHEJ, is associated with a low level of apoptosis of neural cells and premature neurogenesis, which consists of an early shift of neural progenitors from proliferative to neurogenic divisions during brain development. We show that premature neurogenesis is related to an increase in chromatid breaks affecting mitotic spindle orientation, highlighting a direct link between asymmetric chromosome segregation and asymmetric neurogenic divisions. This study reveals thus that XLF is required for maintaining symmetric proliferative divisions of neural progenitors during brain development and shows that premature neurogenesis may play a major role in neurodevelopmental pathologies caused by NHEJ deficiency and/or genotoxic stress.