Trilogy of comprehensive treatment of kitchen waste by bacteria-microalgae-fungi combined system: Pretreatment, water purification and resource utilization, and biomass harvesting.

Given its profound disservice, a bacteria-microalgae-fungi combined system was designed to treat kitchen waste. Firstly, a new type of microbial agent homemade compound microorganisms (HCM) (composed of Serratia marcescens, Bacillus subtilis and other 11 strains) with relatively high bio-security were developed for pretreating kitchen waste, and HCM efficiently degraded 85.2 % cellulose, 94.3 % starch, and 59.0 % oil. HCM also accomplished brilliantly the initial nutrients purification and liquefaction conversion of kitchen waste. Under mono-culture mode (fungi and microalgae were inoculated separately in the pre - and post-stages) and co-culture mode (fungi and microalgae were inoculated simultaneously in the early stage), microalgae-fungi consortia were then applied for further water purification and resource utilization of kitchen waste liquefied liquid (KWLL) produced in the pretreatment stage. Two kinds of microalgae-fungi consortia (Chlorella sp. HQ and Chlorella sp. MHQ2 form consortia with pellet-forming fungi Aspergillus niger HW8-1, respectively) removed 79.5-83.0 % chemical oxygen demand (COD), 44.0-56.5 % total nitrogen (TN), 90.3-96.4 % total phosphorus (TP), and 64.9-71.0 % NH4+-N of KWLL. What's more, the microalgae-fungi consortia constructed in this study accumulated abundant high-value substances at the same time of efficiently purifying KWLL. Finally, in the biomass harvesting stage, pellet-forming fungi efficiently harvested 81.9-82.1 % of microalgal biomass in a low-cost manner through exopolysaccharides adhesion, surface proteins interaction and charge neutralization. Compared with conventional microalgae-bacteria symbiosis system, the constructed bacteria-microalgae-fungi new-type combined system achieves the triple purpose of efficient purification, resource utilization, and biomass recovery on raw kitchen waste through the trilogy strategy, providing momentous technical references and more treatment systems selection for future kitchen waste treatment.