World journal of urology · 2025
Rolipram prevents detrusor underactivity related to pelvic nerve injury in rats by promoting regeneration of nerve axons.
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Imai Hitomi, Akaihata Hidenori, Natsuya Hiroki, Harigane Yuki, Yaginuma Kei, Meguro Satoru, Honda-Takinami Ruriko, Matsuoka Kanako, Hoshi Seiji, Hata Junya, Sato Yuichi, Ogawa Soichiro, Kojima Yoshiyuki
Аннотация
PURPOSE: Evaluation of the effects of rolipram, a phosphodiesterase 4 (PDE4) inhibitor, on detrusor underactivity in a rat model of pelvic nerve crush (PNC) injury.
MATERIALS AND METHODS: Female Sprague-Dawley rats (12 weeks old) were divided into sham, PNC, PNC with saline (PNC/Vehicle) and PNC with oral rolipram (1.0 mg/kg/day) treatment (PNC/Rolipram) groups. Cystometrograms, organ bath studies and histological examinations were performed 10 days later.
RESULTS: Micturition interval, bladder capacity and residual volume were significantly increased, and voiding efficiency was significantly decreased in the PNC and PNC/Vehicle groups as compared with the sham group (P < 0.01 each). In organ bath studies, the responses of muscle strips to electrical field stimulation, which activates nerves, were significantly weaker in the PNC and PNC/Vehicle groups than the sham group. Significant improvements in cystometric parameters and muscle strip contractility were seen in the PNC/Rolipram group as compared with the PNC and PNC/Vehicle groups. Immunohistochemical staining demonstrated a significantly increased density of GAP-43-positive nerves, a marker of nerve regeneration, in the detrusor layer of PNC (P = 0.01) and PNC/Vehicle (P = 0.01) groups compared with the sham group. Moreover, the PNC/Rolipram group showed a further significant increase in GAP-43 expression compared with both the PNC group (P = 0.04) and the PNC/Vehicle group (P = 0.04).
CONCLUSIONS: Chronic rolipram treatment might prevent detrusor underactivity by promoting regeneration of nerve axons in a rat PNC model.
MATERIALS AND METHODS: Female Sprague-Dawley rats (12 weeks old) were divided into sham, PNC, PNC with saline (PNC/Vehicle) and PNC with oral rolipram (1.0 mg/kg/day) treatment (PNC/Rolipram) groups. Cystometrograms, organ bath studies and histological examinations were performed 10 days later.
RESULTS: Micturition interval, bladder capacity and residual volume were significantly increased, and voiding efficiency was significantly decreased in the PNC and PNC/Vehicle groups as compared with the sham group (P < 0.01 each). In organ bath studies, the responses of muscle strips to electrical field stimulation, which activates nerves, were significantly weaker in the PNC and PNC/Vehicle groups than the sham group. Significant improvements in cystometric parameters and muscle strip contractility were seen in the PNC/Rolipram group as compared with the PNC and PNC/Vehicle groups. Immunohistochemical staining demonstrated a significantly increased density of GAP-43-positive nerves, a marker of nerve regeneration, in the detrusor layer of PNC (P = 0.01) and PNC/Vehicle (P = 0.01) groups compared with the sham group. Moreover, the PNC/Rolipram group showed a further significant increase in GAP-43 expression compared with both the PNC group (P = 0.04) and the PNC/Vehicle group (P = 0.04).
CONCLUSIONS: Chronic rolipram treatment might prevent detrusor underactivity by promoting regeneration of nerve axons in a rat PNC model.