[Study on secondary metabolites of endophytic fungus Arthrinium sp. A092 from Uvaria microcarpa].

OBJECTIVE

To study the secondary metabolites of the endophytic fungus Arthrinium sp. A092 from Uvaria microcarpa.

METHODS

The compounds were isolated and purified by silica gel column chromatography, reverse silica gel column chromatography, Sephadex LH-20 column chromatography, HPLC, preparative TLC and recrystallization. Their structures were identified by extensive analysis of their spectroscopic data.

RESULTS

Ten compounds were isolated from the fermentation broth extract of strain A092 and identified as flemingipanic acid(1), ( - )-gynuraone(2),2-(4-methoxy-phenyl)-ethanol(3),2-hexyl-methymaleic anhydride(4), {1-[(12E, 16E)]-12,16-eicosadienoyl]-2-[ (E, E)-7, 10-octadecadienoyl]-3-stearoylglycerol} (5), mycoediketoperazine (6), libertellenone C(7),4-hydroxymethyl-4, 6-octadiene-2, 3-diol (8), dimethyl phthalate (9), and di-(2-ethyl)-hexylphthalate (10).

CONCLUSION

Compounds 1,3 - 5 and 8 - 10 are isolated from the genus Arthrinium for the first time.