[Inhibiting effect of Shuang-huang-lian microemulsion on cytokines of carrageenan induced pleuritis in rats].

OBJECTIVE

To explore the anti-inflammatory effect and possible mechanism of Shuang-huang-lian (SHL) microemulsion.

METHOD: Rat model of pleuritis was established by thoracic injecting 0.2 mL of 1% carrageenan. Rats in the treated groups were orally administered with SHL microemulsion prescription 1, 2, and oral liquid, while those in the positive control group were given aspirin. Rats in the normal group and the model group were given equal volume of water. Each groups were given their medicine for successive 6 days. Modeling was performed 30 mins after the 5th day medication. After 12 hrs of modeling, took suction of the pleurorrhea and measured the amount of tumor necrosis factor alpha (TNF-alpha), interleukin-8 (IL-8), prostaglandin E2 (PGE2) and protein (pro).

RESULT: Compared with the normal group, all the parameters were higher in model group (TNF-alpha and IL-8 P<0.01, PGE2 and pro P<0.05). While compared with the model group, only the amount of TNF-alpha and PGE2 were lower in all the treated group (P<0.01).

CONCLUSION

Both SHL microemulsion prescription 1 and 2 have obvious anti-inflammatory effect. The effect might be related to inhibiting the increase of cytokines as TNF-alpha and PGE2, and intervening of the metabolic process of arachidonic acid (AA).