International journal of pediatric otorhinolaryngology · 2021
The heterogeneity of fibroblasts in laryngotracheal stenosis and skin hypertrophic scar in pediatric patients.
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Hu Bin, Wang Jing, Chen Jiarui, Zhao Limin, Li Xiaoyan
Аннотация
OBJECTIVES: To investigate the heterogeneity between the laryngotracheal stenosis and hypertrophic scar derived fibroblasts.
METHODS: Human laryngotracheal stenosis (LTS) and skin hypertrophic scar (HTS) specimens were obtained during the tracheal resection and T-shaped tracheal stent implantation surgery. Fibroblasts were isolated and cultured. Cell proliferation and migration were analyzed by cell count, EdU proliferation and wound-healing assays. The expressions of COL1a1, α-SMA, TGF-β1 signaling pathway, chemokines and receptors were analyzed by qRT-PCR, western blotting, and immunohistochemistry.
RESULTS: Cell proliferation and migration of LTS derived fibroblasts were significantly faster than HTS fibroblasts, with no significant difference of the percentage of apoptotic cells. COL1a1, α-SMA, and Integrins were down-regulated in LTS fibroblasts, but TGFB1 and chemokine receptor CXCR7 were up-regulated in LTS fibroblasts. However, the expressions of SMAD4 and phospho-SMAD2/3 were not significantly different.
CONCLUSIONS: Human LTS and HTS derived fibroblasts differ in cell proliferation and migration. Different expressions of COL1a1, α-SMA, and CXCR7 were found between the two fibroblasts.
METHODS: Human laryngotracheal stenosis (LTS) and skin hypertrophic scar (HTS) specimens were obtained during the tracheal resection and T-shaped tracheal stent implantation surgery. Fibroblasts were isolated and cultured. Cell proliferation and migration were analyzed by cell count, EdU proliferation and wound-healing assays. The expressions of COL1a1, α-SMA, TGF-β1 signaling pathway, chemokines and receptors were analyzed by qRT-PCR, western blotting, and immunohistochemistry.
RESULTS: Cell proliferation and migration of LTS derived fibroblasts were significantly faster than HTS fibroblasts, with no significant difference of the percentage of apoptotic cells. COL1a1, α-SMA, and Integrins were down-regulated in LTS fibroblasts, but TGFB1 and chemokine receptor CXCR7 were up-regulated in LTS fibroblasts. However, the expressions of SMAD4 and phospho-SMAD2/3 were not significantly different.
CONCLUSIONS: Human LTS and HTS derived fibroblasts differ in cell proliferation and migration. Different expressions of COL1a1, α-SMA, and CXCR7 were found between the two fibroblasts.